PET/EVOH nonwoven fabrics support <i>in vitro</i> expansion of mesenchymal stem cells with high differentiation potential
Chen YM., Tokoda C., Tabata Y.
Laboratory Study, published in Regen Ther (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Regen Ther (2026)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41445975
- PMCID
- PMC12722978
- DOI
- 10.1016/j.reth.2025.101046
Abstract (original English)
Cell expansion under three-dimensional (3D) condition has been shown to better preserve cellular properties by mimicking the native microenvironment, thereby creating a more physiologically relevant culture system. This study investigated the capacity of a three-dimensional nonwoven polyethylene terephthalate (PET)/ethylene vinyl alcohol (EVOH) scaffold as a 3D substrate for large-scale expansion of mesenchymal stem cells (MSC). A seven-fold increase in cell number was observed after 14 days of cultivation, and cells were well distributed with an efficient infiltration within scaffolds based on the hematoxylin and eosin (H&E) staining. A high cell survival and pertaining of metabolic activity were demonstrated by live/dead and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) stainings, respectively. The preservation of MSC properties was confirmed by their differentiation potential toward osteogenic, adipogenic, and chondrogenic lineages. Both in situ differentiation of cell-expanded scaffolds and the subsequent differentiation after cell retrieving from scaffolds revealed the successful responsiveness of expanded MSC to lineage-specific stimuli. These findings suggest the potential of this PET/EVOH scaffold as the 3D culture substrate enabling efficient MSC proliferation while maintaining key functional properties.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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