Phosphatase and tensin homolog accelerate the inflammatory injury and glycolysis of chondrocytes through TP53 protein-mediated p38MAPK pathway activation in a high-glucose environment

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objective Chronic metabolic diseases represent a high-risk factor for osteoarthritis. In particular, diabetes has been proven to be closely related to the occurrence of osteoarthritis in cases called diabetic osteoarthritis (DAO). A large number of studies have shown that phosphatase and tensin homolog (PTEN) are involved in the progression of osteoarthritis; however, the mechanism of PTEN in DAO remains unclear. Accordingly, this study aimed to explore the regulatory role of PTEN in inflammatory injury and glycolysis during the progression of DAO. Material and methods Primary chondrocytes treated with high glucose (HG) were transfected with the small interfering (si) RNA of PTEN or tumor suppressor protein p53 (TP53). Then, cell viability and apoptosis, inflammatory factor secretion, and the protein expression of matrix metalloproteinase 13 and collagen II were detected. Subsequently, we examined glucose uptake, lactate production, reactive oxygen species (ROS) content, and the protein expression of glycolysis-related molecules, namely hexokinase2 and lactate dehydrogenase A, in PTEN knockdown primary chondrocytes. Then, primary chondrocytes were transfected with siRNA against PTEN (siPTEN) alone or together with plasmid DNA (pcDNA)-TP53 to detect changes in cellular inflammatory damage and glycolysis, and thus, determine whether the p38 mitogen-activated protein kinase (p38MA

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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