Photo-Triggered Hyaluronic Acid Hydrogel for 3D Culture and Cell Recovery.
Palmieri F., Gueye M., Del Rio LV., Bunschuh S., Chopra P., Mihăilă S.
Laboratory Study, published in Chembiochem (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Chembiochem (2025)
- Country
- Germany
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40601287
- PMCID
- PMC12631004
- DOI
- 10.1002/cbic.202500310
Abstract (original English)
Properties of semisynthetic hydrogels can be fine-tuned, making these attractive for various applications in regenerative medicine. Herein, a hydrogel platform based on hyaluronic acid (HA) modified by (1R, 8S,9S)-bicycle[6.1.0]non-4-yn-9-ylmethanol (BCN) and a cross-linker composed of light-sensitive o-nitrobenzyl and polyethylene glycol (PEG) chains terminating in azides is described. The two components can undergo strain-promoted azide-alkyne cycloaddition resulting in rapid gel formation. First, adipose-derived mesenchymal stromal cells (MSCs) are incorporated in the hydrogel and it is demonstrated that the cells can be easily retrieved by UV light-mediated degradation, maintaining viability and retaining spindle-like shape when the cells are replated. Next, a proof-of-concept of inducing light-mediated softening of the hydrogel to modulate the morphology of the encapsulated cells is provided. A co-culture of endothelial cells (cord blood-derived endothelial colony forming cells and bone marrow-derived MSCs), which is commonly studied for their ability to form capillary-like vascular networks, is cultured in the regular and light-induced softened hydrogels. Nonphotoexposed hydrogels show cells with a prevalently rounded morphology, whereas stretched cells connecting into a primitive capillary network are observed in the light-softened hydrogels. Photo-induced softening offe
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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