Photobiomodulation-induced Differentiation of Adipose-derived Stem Cells into Neuronal Organoid-like Structures.
Mulaudzi PE., Abrahamse H., Crous A.
Animal Study, published in Mol Neurobiol (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Mol Neurobiol (2026)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42159819
- PMCID
- PMC13190451
- DOI
- 10.1007/s12035-026-05903-y
Abstract (original English)
The ability to regulate stem cell differentiation into organized neural tissue remains a major challenge in regenerative medicine, particularly in the development of physiologically relevant three-dimensional (3D) organoid models. Photobiomodulation (PBM) is a new non-invasive technology for controlling cellular metabolism and differentiation using light-mediated signalling pathways; however, its role in neural organoid development remains insufficiently understood. This study investigated the effects of PBM on the differentiation of adipose-derived mesenchymal stem cells (ADMSCs) into neuronal organoid-like structures using a 3D culture system. Immortalized ADMSCs were exposed to 525 nm and 825 nm wavelengths, individually and in combination, at fluences of 5 and 10 J/cm 2 . PBM's effects were assessed using morphological assessment, cell viability, ATP-based metabolic activity, mitochondrial membrane potential (ΔΨm), and neural gene expression. PBM treatment affected organoid morphology, metabolic activity, and mitochondrial function in a dose- and wavelength-dependent way. Low-fluence irradiation (5 J/cm 2 ), especially at 525 nm, promotes stem cell maintenance and early neural development, as evidenced by enhanced expression of progenitor and neuronal markers. Higher fluence (10 J/cm 2 ) inhibited early differentiation responses, but mixed wavelength therapy promoted late-s
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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