Plant-derived extracellular vesicles quality control: key process progress and future research directions
Shu F., Hu Y., Sarsaiya S., Jin L., Yang X., Liu F.
Narrative Review, published in Discov Nano (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Narrative Review
- Journal
- Discov Nano (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41400892
- PMCID
- PMC12708502
- DOI
- 10.1186/s11671-025-04399-0
- Citations
- 9
Abstract (original English)
Plant-derived extracellular vesicles (PDEVs) have garnered significant attention as crucial mediators of intercellular and cross-species communication in plants, owing to their unique bioactivity and medicinal potential. However, quality control of PDEVs is of paramount importance for their functionality and applications. This review systematically summarizes the key aspects that influence PDEVs quality, including source material preparation, isolation and purification, characterization, and storage conditions. Regarding source material preparation, the genetic background, abiotic factors (such as light, temperature, and moisture), biotic factors (including pathogen infection and probiotic effects), and traditional processing methods significantly affect the composition and function of PDEVs. Plant tissue culture techniques and temporary immersion bioreactor systems (TIBS) have substantial potential for addressing the issue of fluctuating raw material quality. During the isolation and purification processes, existing methods (e.g., ultracentrifugation, polymer precipitation, and size-exclusion chromatography) have their respective advantages and disadvantages, necessitating the optimization of operations based on specific requirements to avoid vesicle damage or impurity introduction. In terms of characterisation, integrating techniques such as transmission electron microscopy (
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is a narrative review: it collects no new patient data and does not systematically appraise evidence quality.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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