Platelet-rich fibrin-conditioned medium promotes osteogenesis of dental pulp stem cells through TGF-β and PDGF signaling
Fujii Y., Yoshida T., Sato A., Ikehata M., Hatori A., Chikazu D.
Laboratory Study, published in Regen Ther (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Regen Ther (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 40511256
- PMCID
- PMC12159295
- DOI
- 10.1016/j.reth.2025.05.006
- Citations
- 1
Abstract (original English)
Introduction Mesenchymal stem cells (MSCs), a key cell source for regenerative medicine, are conventionally cultured in human autologous serum (HAS) or fetal bovine serum (FBS). Platelet-rich fibrin-conditioned medium (PRF-CM) has emerged as a potential alternative to FBS, promoting the osteogenic differentiation of dental pulp stem cells (DPSCs) more efficiently than FBS. However, the molecular mechanisms underlying PRF-CM-induced osteogenesis in DPSCs remain unclear. Therefore, the aim of this study was to elucidate these mechanisms. Methods PRF-CM was prepared from peripheral blood samples collected from three healthy donors. DPSCs were derived from the dental pulp extracted from the third molars of three healthy donors. The mRNA expression patterns of DPSCs cultured in FBS or PRF-CM were compared using RNA sequencing (RNA-Seq). Differentially expressed genes (DEGs) were analyzed by Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Results The mean blood volume collected from the three donors was 51.3 mL, yielding a mean PRF-CM volume of 168 mL. RNA-Seq identified 2258 DEGs, and KEGG pathway and GO analysis revealed that PRF-CM upregulated biological activities associated with transforming growth factor beta (TGF-β) signaling and platelet-derived growth factor (PDGF) binding compared with FBS. Western blotting analysis reveale
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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