Platelet-rich plasma enhances local homing of umbilical cord-derived mesenchymal stem cells to articular cartilage by increasing the quantity and activation of integrin ꞵ1

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Enhancing mesenchymal stem cells (MSCs) engraftment at the degenerative cartilage is important to increase the therapeutic effect of cartilage regeneration. Platelet-rich plasma (PRP) is known to have anti-inflammatory and anabolic effects for the treatment of osteoarthritis and has been reported to be commonly used with MSCs. However, little is known about the effects of PRP on MSCs adhesion to cartilage extracellular matrix (ECM). The purpose of this study was to investigate how PRP pre-conditioning enhances MSC adhesion to cartilage ECM and improves the efficacy of MSCs in promoting cartilage regeneration in a rat osteochondral defect model. Method In vitro adhesion of umbilical cord-derived MSCs (UC MSCs) to collagens, fibronectin, and hyaluronic acid and to osteochondral explants was measured with or without PRP pre-conditioning using a cell viability assay. The mRNA expression of integrin subunits and the activity level of integrin ꞵ1 (ITGB1) were evaluated using RT-PCR and western blot, respectively. After establishing an osteochondral defect in the femoral trochlea in Sprague-Dawley rats (n = 26), UC MSCs with PRP were locally injected into the defect. After 4 weeks, macroscopic and histological evaluations were performed using ICRS and O'Driscoll scoring systems. Results PRP significantly increased the adhesion of MSCs to collagens, fibronectin, and degenera

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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