SVF
Level D· Preclinical EvidenceLaboratory Study

Polylactic Acid Constructs Induce Metabolic and Proteomic Changes in the Secretome of Adipose-derived MSCs.

Barisón MJ., Bandini TB., Berti LF., Stimamiglio M., Correa A.

Laboratory Study on Face & Skin, published in Stem Cell Rev Rep (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Laboratory Study
Journal
Stem Cell Rev Rep (2026)
Country
United States
Reported sample size
—
PMID
41286394
DOI
10.1007/s12015-025-11020-5

Abstract (original English)

Tissue engineering seeks to develop biomimetic substitutes for damaged tissues using natural or synthetic materials functionalized with cells or biologically active compounds. Understanding how cell-scaffold interactions influence cellular behavior is critical for optimizing tissue engineering strategies. Polylactic acid (PLA), a biodegradable and biocompatible polymer approved by the U.S. FDA, is commonly used for scaffolds fabrication. Mesenchymal stromal cells (MSCs) are widely utilized in regenerative medicine due to their capacity to differentiate into mesodermal lineages and their secretomes, which exhibit robust paracrine activity. To date, few studies have investigated how scaffold characteristics, such as materials and/or architecture, modulate secretome composition using integrated multi-omics approaches. An untargeted metabolomics workflow combined with label-free proteomics was employed to analyze ASC-derived secretomes, obtained from adipose tissue-derived MSCs (ASCs) cultured in PLA-discs and PLA-scaffolds, as well as on conventional 2D polystyrene (PS) culture surfaces. A similar proteomic distribution was observed when secretomes from PLA-discs and PLA-scaffolds were compared. On the other hand, significant changes in the proteomic profile were observed between PLA-discs and 2D-PS secretomes. Proteins belonging to carbohydrate metabolism, cell motility, vasculat

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence
Mesenchymal Stem CellsPolyestersHumansAdipose TissueProteomicsSecretomeTissue ScaffoldsTissue EngineeringCells, CulturedCell Differentiation
Face & Skin
View on PubMedDOI

Related research