The potential neuroprotective effect of human adipose stem cells conditioned medium against light-induced retinal damage.
Sugitani S., Tsuruma K., Ohno Y., Kuse Y., Yamauchi M., Egashira Y.
Animal Study, published in Exp Eye Res (2013) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Exp Eye Res (2013)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 24076412
- DOI
- 10.1016/j.exer.2013.09.013
- Citations
- 29
Abstract (original English)
Human adipose-derived stem cells (hASCs) are present in adult adipose tissue and have been reported to secrete various factors that have neuroprotective effects. In the present study, we examined whether hASC-conditioned medium (hASC-CM) was effective against experimental degenerative retinal disease. Mature adipocytes (MAs) and hASCs were isolated from human subcutaneous adipose tissue. The isolated hASCs were identified based on their capacity for bone and neural differentiation. The effects of hASC-CM against tunicamycin-, H2O2-, and light-induced retinal photoreceptor damage were evaluated in vitro by measuring cell death. Moreover, we identified various factors present in hASC-CM using antibody arrays. Retinal damage induced in mice by exposure to white light was studied in vivo, and photoreceptor damage was evaluated according to the thickness of the outer nuclear layer and electroretinography results. In addition, the effect of hASC-CM on Akt phosphorylation at Ser473 was confirmed by western blotting. Finally, the effects of the secreted proteins identified in the hASC-CM on light-induced damage were evaluated in vivo. Isolated hASCs differentiated to osteocytes and neurons. hASC-CM protected against tunicamycin-, H2O2-, and light-induced cell death. In addition, hASC-CM inhibited photoreceptor degeneration and retinal dysfunction after exposure to light. Several protei
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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