The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells.
Ji F., Wang Y., Yuan J., Wu Q., Wang J., Liu D.
Laboratory Study, published in J Cell Physiol (2019) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- J Cell Physiol (2019)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 31566725
- DOI
- 10.1002/jcp.29243
- Citations
- 11
Abstract (original English)
To investigate the potential role of stromal cell-derived factor-1α (SDF-1α)/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells (ADSCs), quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed to screen the effective small interfering RNA against CXCR4 and CXCR7 in ADSCs. The messenger RNA (mRNA) and proteins abundances of AKT (p-AKT), ERK (p-ERK), JNK (p-JNK), and p38 (p-p38) in different groups were identified by qRT-PCR, western blot, and immunofluorescence staining method. Meanwhile, cell migration and cell proliferation with SDF-1 treated were examined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and transwell permeable assay, respectively. Moreover, the interaction between CXCR4 and CXCR7 was examined by a GST pull-down assay. CXCR4 small interfering RNA3 (siRNA3) and CXCR7 siRNA3 have been proved to the most effective tools for knockdown CXCR4 and CXCR7 expressions. mRNA abundance of JNK and p38 could be affected by SDF-1α/CXCR4/CXCR7 axis. However, western blot analysis of p-AKT, p-ERK, p-JNK, and p-p38 in CXCR43-treated ADSCs was significantly higher than that in the control group. Moreover, the immunofluorescence staining analysis revealed that the expressions of p-ATK and p-JNK proteins were significantly higher in NC- and SDF-1-treated subgroups than that in the CXCR4 and CXCR7 groups. p-ATK and p-JNK pr
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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