Practical Method for Excised Adipose Tissue Cryopreservation Using Commercial Freezers: Optimized Thawing and Evaluation of Isolated SVF Cells.
Farhana S., Shamsuddin SH., Wan Sulaiman WA., Kadir R., Mohd Nasir NA.
Laboratory Study, published in Plast Reconstr Surg (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Plast Reconstr Surg (2026)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42117696
- DOI
- 10.1097/PRS.0000000000013174
Abstract (original English)
Background Adipose tissue is vital for plastic and reconstructive surgery due to its accessibility and regenerative potential via abundant stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs). However, unpredictable graft resorption remains a major limitation, for which cryopreservation offers a potential solution. Yet, cryopreserving excised adipose tissue in commercial freezers with mechanical isolation of ADSCs remains unexplored. Methods Human adipose tissue from 22 donors was preserved as tissue blocks or lipoaspirates at -20 °C or -80 °C for up to 4 months. Across 284 technically replicated experiments, oil release, mitochondrial activity (XTT assay), SVF yield and viability (trypan blue and flow cytometry), and ADSC multilineage differentiation were assessed. Fresh samples served as controls. Results Rapid thawing at 37 °C (thawing method 3) minimized oil leakage (0.02 ml) and better preserved SVF yield with viability (3.03 and 0.64 × 10 4 cells/ml) after 2 months of tissue block preservation at -80 °C compared with lipoaspirates, per gram of fat. After 4 months, -80 °C-stored samples retained more SVF cells (88%) with 68% viability and showed stronger ADSC marker expression (CD73⁺/CD90⁺/CD105⁺: 70-80%) than -20 °C samples. ADSCs from -80 °C stored blocks differentiated into adipogenic, chondrogenic, and osteogenic lineages, whereas -20 °C stored sample
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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