[Preliminary comparison study of adipogenic differentiation capacity between dedifferentiated adipocytes cells and adipose-derived stem cells in vivo].
Chen XW., Jiang P., Gao JH., Liao YJ., Lu F.
Animal Study with a reported sample of 8, published in Zhonghua Zheng Xing Wai Ke Za Zhi (2010) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Zhonghua Zheng Xing Wai Ke Za Zhi (2010)
- Country
- China
- Reported sample size
- 8
- Source database
- PubMed
- PMID
- 21174796
Abstract (original English)
Objective To compare the adipogenic differentiation capacity of dedifferentiated adipocytes cells (DA) and adipose-derived stem cells (ASCs) in vivo, so as to select good adipogenic seed cells for tissue engineering. Methods Mature adipocytes and ASCs were isolated by means of enzymatic digestion from the liposuction aspirate. Then the DA cells were acquired by ceiling adherent culture of mature adipocytes and the 3rd passage cells were used. The DA cells and ASCs were cultured with fibrin glue in vitro respectively. The compatibility of scaffold with cells was detected by microscopy and scanning electron microscopy. The scaffold-cell composite was also labeled by DiI. The composite was injected subcutaneously on the nude mice back, respectively (DA-FG group, n = 8; ASCs-FG group, n = 8; sham FG group, n = 8). 8 weeks after implantation, the newly formed tissue was taken out for general observation and histologic study. Results Mature adipocytes were transferred to DA cells with spindle shape, like fibroblast. The ASCs were also spindle. Three days after culture of cell-scaffold composite in vitro, the cells grew well. 8 weeks after implantation, the newly formed tissue was found under the skin both in DA-FG and ASCs-FG groups, but not in sham FG group. The newly formed tissue was mature fat tissue and originated from the seed cells. The average wet weight of the new-formed tis
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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