Production enhancement of human adipose-derived mesenchymal stem cells by low-intensity ultrasound stimulation.
Min S., Byeon Y., Kim M., Lee Y., Lee SH., Lee Y.
Animal Study, published in Sci Rep (2022) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Sci Rep (2022)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 36543825
- PMCID
- PMC9772213
- DOI
- 10.1038/s41598-022-24742-0
- Citations
- 8
Abstract (original English)
Abstract Background: Low-intensity ultrasound (LIUS) has been used to increase the proliferation rate of various stem cells including human adipose-derived mesenchymal stem cells (hAdMSCs). hAdMSCs is now commercially produced for various therapeutic applications. The purpose of this study was to show feasibility of enhancing the productivity of cell culture during 16-day cell culturing and increasing proliferation rate of hAdMSCs by LIUS stimulation with appropriate ultrasound parameters. Methods: Beam patterns of 5 and 10 MHz ultrasound transducers were measured to confirm the area of stimulation. The intensity of sound waves transmitted through a Petri-dish was measured in situ for quantitative evaluation. Bromodeoxyuridine (BrdU) incorporation assay was performed to search for appropriate parameters for LIUS stimulation of hAdMSCs. Cell culture medium supplemented with 8% fetal bovine serum (FBS) in a 35 mm Petri-dish was used for 16 days with subculture from 2 to 6 passage. Results: A frequency of 5 MHz, an intensity of 300 , a duration of 10 minutes per day, and continuous waves with 100% duty cycle were the best parameters according to the BrdU assay of proliferation rate of hAdMSCs. LIUS stimulation group had about 3.25-fold greater number of cells from passage 2 to 6 compared with the control group. Doubling time was decreased to 4.44 hours in average. Cell viability w
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.