Proliferation and differentiation of rat adipose precursor cells in chemically defined medium: differential action of anti-adipogenic agents.
Vassaux G., Négrel R., Ailhaud G., Gaillard D.
Animal Study, published in J Cell Physiol (1994) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Cell Physiol (1994)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 7962109
- DOI
- 10.1002/jcp.1041610209
Abstract (original English)
Primary rat adipose precursor cells, maintained in the minimal chemically defined medium (ITT medium) able to promote differentiation, have been used to investigate the ability of several agents to modulate their proliferation and their differentiation. Fetuin and fibroblast growth factor (FGF), which exhibited a strong and a weak mitogenic activity, respectively, do not significantly affect the proportion of differentiated cells as indicated by glycerol-3-phosphate dehydrogenase (GPDH) activity values. In contrast, carbaprostacyclin (cPGI2), a stable analogue of prostacyclin, behaves as a true adipogenic factor leading to a 4 to 5-fold increase in GPDH-specific activities with no significant effect on cell growth. Submaxillary gland kallikrein (SMGK), transforming growth factor-beta (TGF-beta) and tumor necrosis factor-alpha (TNF-alpha) behave as growth-promoting agents but at the same time elicit a dose-dependent inhibition of differentiation. Epidermal growth factor (EGF) and prostaglandin F2 alpha (PGF2 alpha) do not show any effect on cell proliferation at concentrations which exert a maximal inhibitory action on differentiation. Upon removal of EGF from the culture medium, complete resumption of differentiation occurs, whereas upon removal of PGF2 alpha or SMGK, complete resumption only takes place when differentiation is triggered by cPGI2. Upon removal of TNF-alpha, a p
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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