Level D· Preclinical EvidenceLaboratory Study

Promotion of bone-tendon healing after ACL reconstruction using scaffold-free constructs comprising ADSCs produced by a bio-3D printer in rabbit models.

Higa K., Murata D., Azuma C., Nishida K., Nakayama K.

Laboratory Study on Tendon Injury, Ligament Injury, Rotator Cuff, published in J Orthop Translat (2025) — summary generated from the PubMed abstract.

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Study type: Laboratory Study
Journal: J Orthop Translat (2025)
Country: Singapore
Reported sample size: —
PMID: 40342550
DOI: 10.1016/j.jot.2025.03.019

Abstract (original English)

Background/Objective: This study evaluated the impact of adipose tissue-derived mesenchymal stromal cells (ADSCs) on bone-tendon healing in rabbit anterior cruciate ligament (ACL) reconstruction. Nineteen mature male Japanese White rabbits underwent bilateral ACL reconstruction. ADSC constructs were implanted in the right femoral bone tunnel of each rabbit (implant group), while the left knee served as the control group without implantation. Nine rabbits were sacrificed at 3 and 6 weeks post-surgery, while the remaining were sacrificed immediately post-surgery. Biomechanical and micro computed tomography evaluations were conducted on six rabbits, while histological observation was performed on the remaining three. showed: (1) The implant group exhibited a significantly greater failure load than the control group at 3 weeks post-surgery. (2) Initially, the amount of new bone in the femoral tunnel was lower in the implant group at 3 weeks but surpassed that of the control group by 6 weeks. (3) Histological analysis indicated faster bone-tendon healing in the implant group than that of the control. These findings suggest a positive effect of ADSC constructs on bone-tendon healing post-ACL reconstruction in rabbits. However, further studies using larger animal models must confirm these effects comprehensively. The method of transplanting a scaffold-free autologous ADSC construct is

What this study does not prove

• This study does not prove SVF is an approved treatment or a replacement for standard care.
• This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence

Face & Skin Knee Shoulder Tendon Injury Ligament Injury Rotator Cuff

View on PubMed DOI

Promotion of bone-tendon healing after ACL reconstruction using scaffold-free constructs comprising ADSCs produced by a bio-3D printer in rabbit models.

Abstract (original English)

What this study does not prove

Evidence level

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