The promotion of chondrogenesis, osteogenesis, and adipogenesis of human mesenchymal stem cells by multiple growth factors incorporated into nanosphere-coated microspheres.
Park JS., Yang HN., Woo DG., Jeon SY., Park KH.
Animal Study, published in Biomaterials (2010) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Biomaterials (2010)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 20875915
- DOI
- 10.1016/j.biomaterials.2010.08.088
Abstract (original English)
The induction of stem cell differentiation by drugs and growth factors has been the objective of many studies designed to develop methods for the formation of new tissues or the repair of degenerated tissues via transplantation. In this study, drugs and growth factors with high potential for use in tissue repair were embedded in human mesenchymal stem cells (hMSCs), which were then induced to differentiate into chondrogenic, osteogenic, and adipogenic lineages. Additionally, microspheres coated and loaded with the drugs and growth factors successfully proliferated and, as expected, induced the differentiation of transplanted hMSCs into the desired specific cell types. Furthermore, hMSCs transplanted in micro-typed scaffolds prevented changes in differentiation. RT-PCR and western blot analysis of the resultant cartilage, bone, and adipose tissues showed that a combination of drugs and different growth factor types induced the differentiation of transplanted hMSCs. Additionally, histology and immunohistochemistry showed that specific ECMs and proteins released from transplanted hMSCs were present within the nanosphere-coated microspheres. The results of this study show that the regulation of stem cell differentiation by drugs and growth factors might enable the fabrication of therapeutic materials for the delivery of stem cells that are simpler to use, less expensive, and more e
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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