Proteomic and Metabolomic Analyses of Adipose-derived Mesenchymal Stem Cell Exosomes and Culture Supernatants.
Hirakawa T., Kato T., Nakanishi Y., Urushiyama D., Miyata K., Baba T.
Laboratory Study, published in Anticancer Res (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Anticancer Res (2025)
- Country
- Greece
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40750428
- DOI
- 10.21873/anticanres.17703
Abstract (original English)
Background/aim Aberrant differentiation of fertilized eggs during in vitro fertilization is a major contributor to infertility, and adipose-derived mesenchymal stem cells (ASCs) and their exosomes have been reported to facilitate fertilized egg differentiation; however, the underlying mechanisms remain unclear. This study aimed to elucidate how ASC-derived exosomes promote fertilized egg differentiation through proteomic analysis of ASC-derived exosomal proteins and metabolomic profiling of culture supernatants. Materials and methods Extracellular vesicles were isolated from ASC culture supernatants via differential ultracentrifugation, proteins were extracted and digested using a phase-transfer surfactant protocol for LC-MS/MS analysis, and metabolites were analyzed using GC-MS/MS following extraction and derivatization. Results Exosomes from early passage ASCs were enriched with various proteins, including alpha and beta proteasome subunits, which exhibit proteasome activity. Gene ontology analysis revealed the presence of proteins associated with transmembrane transport and cytoplasmic translation. Metabolomic profiling of culture supernatants revealed markedly elevated levels of amino acids associated with glycine, serine, and methionine metabolism. Conclusion ASC-derived culture supernatants can exert differentiation-promoting effects on fertilized eggs via exosomal protei
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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