A Protocol for co-Injecting Cells with Pulverized Fibers for Improved Cell Survival and Engraftment

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Adipose tissue is crucial for medical applications such as tissue reconstruction, cosmetic procedures, and correcting soft tissue deformities. Significant advances in the use of adipose tissue have been achieved through Coleman's studies in fat grafting, which gained widespread acceptance due to its effectiveness and safety. Despite its benefits, adipose tissue grafting faces several limitations, including high absorption rates due to insufficient support or anchorage, replacement by fibrous tissue, migration from the intended site, and loss of the initial desired morphology post-administration. To counteract these constraints, there is a need for improved grafting techniques that enhance the predictability and consistency of outcomes. Biomaterials are extensively used in tissue engineering to support cell adhesion, proliferation, and growth. Both natural and synthetic materials have shown promise in creating suitable microenvironments for adipose tissue regeneration. PLGA, a synthetic copolymer, is particularly notable for its biocompatibility, biodegradability, and tunable mechanical properties. Here, we describe a protocol using milled electrospun poly(lactic-co-glycolic acid) (PLGA) fibers combined with lipoaspirated tissue to create a fibrous slurry for injection. By pulverizing PLGA fiber mats to create fiber fragments with increased pore size and porosity, we can influen

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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