A Protocol to Induce Brown and Beige Adipocyte Differentiation From Murine and Human Adipose-Derived SVF.
Yadav RR., Mishra N., Verma N.
Animal Study, published in Bio Protoc (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Bio Protoc (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41377103
- PMCID
- PMC12689158
- DOI
- 10.21769/BioProtoc.5522
Abstract (original English)
Adipose cells vary functionally, with white adipocytes storing energy and brown/beige adipocytes generating heat. Mouse and human subcutaneous white adipose tissue (WAT)-derived stromal vascular fraction (SVF) provides mesenchymal stem cells (MSCs) that can be differentiated into thermogenic adipocytes using pharmacological cocktails. After six days of browning induction, these cells exhibited significant upregulation of thermogenic markers ( UCP1, Cidea, Dio2, PRDM16 ) along with adipogenic genes ( PPARγ, aP2 ), showing enhanced thermogenic potential. This in vitro system offers a practical platform to study adipogenesis and thermogenic regulation. Key features • The protocol aims to differentiate murine and human SVF from subcutaneous fat into brown/beige adipocytes for assays. • This approach employs a straightforward methodology that effectively differentiates SVF cells using optimal chemicals, their concentrations, and durations.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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