Protocol for in situ transplantation of adipose stem cells into mouse epididymal white adipose tissue.
Liao X., Zeng Q., Zhang H., Xie L., Deng T.
Animal Study, published in STAR Protoc (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- STAR Protoc (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40819366
- PMCID
- PMC12390934
- DOI
- 10.1016/j.xpro.2025.104035
Abstract (original English)
Adipose stem cells (ASCs) play a crucial role in maintaining adipose tissue homeostasis, but studying them in situ is technically challenging. Here, we present a protocol for the transplantation of ASCs into mouse epididymal white adipose tissue (eWAT). We describe steps for isolating and purifying ASCs, labeling them with a cell tracker, administering anesthesia, making an incision, and performing spot injections of ASCs into the eWAT of mice. This protocol offers broad applications for in vivo functional research of ASCs. For complete details on the use and execution of this protocol, please refer to Liao et al. 1 .
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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