Protocol for stromal vascular fraction isolation from inguinal subcutaneous white adipose tissue and beige adipocyte differentiation.
Tang Q., Xu J., Yang X., Li H., Wang L., Zhu L.
Animal Study, published in STAR Protoc (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- STAR Protoc (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41014562
- PMCID
- PMC12509890
- DOI
- 10.1016/j.xpro.2025.104115
Abstract (original English)
Research on inducing beige adipocytes to combat obesity and its comorbidities is growing, with stromal vascular fraction (SVF) from white adipose tissue showing potential to differentiate into both white and beige adipocytes. Here, we present a protocol for isolating the SVF from the iWAT (inguinal white adipose tissue) of mice and differentiating them into mature beige adipocytes. This protocol can be used to analyze the translational regulation and metabolic processes of beige adipocytes in vitro. For complete details on the use and execution of this protocol, please refer to Xu et al. 1 .
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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