PSXV-42 Role of cholesterol in proliferation and differentiation of bovine myoblasts
Hou Y., Jiang H.
Laboratory Study, published in J Anim Sci (2018) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- J Anim Sci (2018)
- Reported sample size
- —
- Source database
- Europe PMC
- PMCID
- PMC6285252
Abstract (original English)
Abstract The objective of this study was to determine the role of extracellular, cytosolic, and membrane cholesterol in proliferation and differentiation of bovine myoblasts. Myoblasts were isolated from five steers and used in three experiments. In the first experiment, myoblasts were cultured with 2% lipoprotein deficient fetal calf serum (LPDS) or normal fetal calf serum (FCS). Culturing with LPDS did not alter the cytosolic or membrane cholesterol content, or myoblast differentiation, but inhibited myoblast proliferation rate by 20% (P < 0.05), compared to culturing with normal FCS. In the second experiment, myoblasts were cultured with or without lovastatin, a selective inhibitor of cholesterol synthesis. Culturing with 5 μM lovastatin did not affect medium concentration of cholesterol, but reduced cytosolic and membrane cholesterol contents by 30 and 40%, respectively, compared to culturing with vehicle control (P < 0.05). Myoblasts treated with lovastatin in growth medium had 45% less (P < 0.05) proliferating cells than did control myoblasts. Myoblasts treated with lovastatin in differentiation medium had less (P < 0.05) mRNA expression of markers of differentiated myoblasts and fused myotubes than control myoblasts. In the third experiment, myoblasts were cultured with or without methyl-β-cyclodextrin (MβCD), a chemical that depletes cholesterol from cell m
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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