Rapid, practical and safe isolation of adipose derived stem cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

We compared migration, proliferation, growth curve, confluency and differentiation into adipogenic, osteogenic and chondrogenic cell lineages of mesenchymal stem cells derived from adipose tissue cultured in scratched and nonscratched cell culture flasks. Mesenchymal stem cells were isolated from rat adipose tissue using a nonenzymatic method. We investigated two groups. For the control group, minced adipose tissue was implanted conventionally onto the surface of standard plastic cell culture flasks. For the experimental group, the tissues were cultured in flasks with a scratched surface. We found that scratched flasks promoted cell migration, proliferation and confluency. Our findings suggest that scratched flasks may be used to ensure rapid, practical and safe isolation of adipose tissue-derived stem cells.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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