[Recombinant human insulin gene lentivirus transfecting human umbilical cord mesenchymal stem cells in vitro].
Liu Y., Xue M.
Laboratory Study, published in Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2010) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2010)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 20695379
Abstract (original English)
To construct the lentiviral vector to co-express enhanced green fluorescent protein (EGFP) gene and human insulin (insulin) gene, and to explore the condition to transfect human umbilical cord mesenchymal stem cells (hUCMSCs) so as to lay a foundation for tissue engineered adipose reconstruction and transplantation in vivo in future. The insulin gene was cloned to lentiviral expression vector with EGFP [pLenti6.3-internal ribosome entry site (IRES)-EGFP] by recombinant DNA technology, the positive clones were screened, and lentiviral packaged systems and target gene plasmid were co-transfected to package virus in 293T cells by lipofectin. The reporter gene expression was observed by fluorescent inverted phase contrast microscope, virus supernatant was collected, purified and concentrated, and the titer of recombinant viruses was determined, hUCMSCs from umbilical cord tissue of mature neonates were isolated and cultured by different multiple of infection (MOI, 0, 1, 3, 5, 7, 10, 15, and 20). By recombinant lentiviral infected hUCMSCs with reporter gene green fluorescent protein expression, the best MOI was screened; recombinant lentiviral infected hUCMSCs at the best MOI, then real-time PCR and Western blot methods were applied to detect insulin gene and insulin protein expression levels in cells. The recombinant lentiviral vector of co-expressing insulin gene and EGFP gene (pL
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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