Regeneration of hyaline-like cartilage and subchondral bone simultaneously by poly(l-glutamic acid) based osteochondral scaffolds with induced autologous adipose derived stem cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Osteochondral tissue engineering is challenged by the difficulty in the regeneration of hyaline cartilage and the simultaneous regeneration of subchondral bone. In the present study, nhydroxyapatite-graft-poly(l-glutamic acid) (nHA-g-PLGA) was prepared by surface-initiated ring-opening polymerization, which was then used to fabricate an osteogenic scaffold (scaffold O) instead of nHA to achieve better mechanical performance. Then, a single osteochondral scaffold was fabricated by combining the poly(l-glutamic acid) (PLGA)/chitosan (CS) amide bonded hydrogel and the PLGA/CS/nHA-g-PLGA polyelectrolyte complex (PEC), possessing two different regions to support both hyaline cartilage and underlying bone regeneration, respectively. Autologous adipose derived stem cells (ASCs) were seeded into the osteochondral scaffold. The chondrogenesis of ASCs in the scaffold was triggered in vitro by TGF-β1 and IGF-1 for 7 days. In vitro, a chondrogenic scaffold (scaffold C) exhibited the ability to drive adipose derived stem cell (ASC) aggregates to form multicellular spheroids with a diameter of 80-110 μm in situ, thus promoting the chondrogenesis while limiting COL I deposition when compared to ASCs adhered in scaffold O. Scaffold O showed the ability to bind abundant BMP-2. Osteochondral scaffolds with induced ASC spheroids in scaffold C and bonded BMP-2 in scaffold O were transplanted into

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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