Regenerative engineered vascularized bone mediated by calcium peroxide.
Daneshmandi L., Laurencin CT.
Animal Study, published in J Biomed Mater Res A (2020) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Biomed Mater Res A (2020)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 31925886
- DOI
- 10.1002/jbm.a.36879
- Citations
- 24
Abstract (original English)
One of the main challenges hindering the clinical translation of bone tissue engineering scaffolds is the lack of establishment of functional vasculature. Insufficient vascularization and poor oxygen supply limit cell survival within the constructs resulting in poor osseointegration with the host tissue and eventually leading to inadequate bone regeneration. Inspired by cues from developmental biology, we regenerative engineered a composite matrix by incorporating calcium peroxide (CaO 2 ) into poly(lactide-co-glycolide) (PLGA) microsphere-based matrices and sought to assess whether the delivery of the byproducts of CaO 2 decomposition, namely O 2 , Ca 2+ , and H 2 O 2 could enhance the regeneration of vascularized bone tissue. The composite microspheres were successfully fabricated via the oil-in-water emulsion method. The presence and encapsulation of CaO 2 was confirmed using scanning electron microscopy, energy dispersive x-ray spectroscopy, thermogravimetric analysis, and X-ray powder diffraction. The microspheres were further heat sintered into three-dimensional porous scaffolds and characterized for their degradation and release of byproducts. The in vitro cytocompatibility of the matrices and their ability to support osteogenic differentiation was confirmed using human adipose-derived stem cells. Lastly, an in vivo study was performed in a mouse critical-sized calvarial
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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