Rehabilitation of N, N'-methylenebisacrylamide-induced DNA destruction in the testis of adult rats by adipose-derived mesenchymal stem cells and conditional medium.
Atia MM., Badr El-Deen AA., Abdel-Tawab HS., Alghriany AAI.
Laboratory Study, published in Heliyon (2024) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Heliyon (2024)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 39669145
- PMCID
- PMC11636104
- DOI
- 10.1016/j.heliyon.2024.e40380
- Citations
- 2
Abstract (original English)
Environmental pollutant acrylamide has toxic effect on human health. Numerous industries such as the paper, and cosmetics, use acrylamide in their manufacturing. In certain foods, acrylamide arises at extremely high temperatures. Mesenchymal stem cells can shield different tissues from the damaging effects of free radicals induced by acrylamide. This study aimed to compare the therapeutic efficacy against acrylamide-induced toxicity between adipose-derived mesenchymal stem cells (MSCs) and their conditioned media (CM), evaluating which is more effective. Seventy adult male rats were employed in this study, distributed among 5 groups. The control group consisted of 10 rats, while each of the other four groups comprised 15 rats. The AC group received a daily oral acrylamide (AC) dosage of 3 mg/kg. In the AC + AD-MSCs and AC + AD-MSCs CM groups, after 4 weeks of AC administration, rats were injected with 0.65 × 10 6 AD-MSCs/0.5 ml PBS and 0.5 ml of AD-MSCs CM, respectively, via the caudal vein, and were observed for 15 days. The recovery group (Rec.), subjected to 4 weeks of AC treatment, and was allowed an additional 15 days for recuperation. The result in AC and Rec. groups revealed elevated DNA damage, P53 protein levels, apoptosis, LPO, and testosterone (free and total). In contrast, the administration of CM and the transplanting of AD-MSCs decreased the levels of these protei
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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