Relative contributions of adipose-resident CD146 + pericytes and CD34 + adventitial progenitor cells in bone tissue engineering.
Wang Y., Xu J., Chang L., Meyers CA., Zhang L., Broderick K.
Animal Study, published in NPJ Regen Med (2019) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- NPJ Regen Med (2019)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 30622740
- PMCID
- PMC6323123
- DOI
- 10.1038/s41536-018-0063-2
- Citations
- 47
Abstract (original English)
Pericytes and other perivascular stem/stromal cells are of growing interest in the field of tissue engineering. A portion of perivascular cells are well recognized to have MSC (mesenchymal stem cell) characteristics, including multipotentiality, self-renewal, immunoregulatory functions, and diverse roles in tissue repair. Here, we investigate the differential but overlapping roles of two perivascular cell subsets in paracrine induction of bone repair. CD146 + CD34 - CD31 - CD45 - pericytes and CD34 + CD146 - CD31 - CD45 - adventitial cells were derived from human adipose tissue and applied alone or in combination to calvarial bone defects in mice. In vitro, osteogenic differentiation and tubulogenesis assays were performed using either fluorescence activated cell sorting-derived CD146 + pericytes or CD34 + adventitial cells. Results showed that CD146 + pericytes induced increased cord formation in vitro and angiogenesis in vivo in comparison with patient-matched CD34 + adventitial cells. In contrast, CD34 + adventitial cells demonstrated heightened paracrine-induced osteogenesis in vitro. When applied in a critical-size calvarial defect model in NOD/SCID mice, the combination treatment of CD146 + pericytes with CD34 + adventitial cells led to greater re-ossification than either cell type alone. In summary, adipose-derived CD146 + pericytes and CD34 + adventitial cells display f
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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