Release kinetics, static and dynamic water contact angles and FTIR data for tissue inhibitor of matrix metalloprotease-1 (TIMP-1) incorporated in electrospun random DegraPol® fibers and TIMP-1 impact on tenocytes and adi

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

A first data set refers to tissue inhibitor of matrix metalloprotease-1 (TIMP-1) protein inclusion into a DegraPol® fibres utilizing emulsion electrospinning and the characterization of the random fibre mesh. Specifically, the release kinetics of the protein from the mesh was studied over 7 days. Moreover, the static and the dynamic water contact angles were determined. Finally, we assessed Fourier-Transformed Infrared Spectra (FTIR spectra) for DegraPol® with and without TIMP-1. A second data set represents proliferation data obtained with the Alamar Blue Assay, applied on rabbit Achilles tenocytes and rabbit adipose-derived stem cells, when stimulated in vitro with 1, 10, and 100 ng/mL TIMP-1 supplementation compared to the corresponding cell culture without TIMP-1 (control). Furthermore, qPCR was performed and collagen I, ki67, tenomodulin and alkaline phosphatase gene expression data are presented for both cell types in vitro stimulated with 1, 10, and 100 ng/mL TIMP-1 supplementation, respectively, and data are presented as manifold induction compared to a TIMP-1-free cell culture medium (control).

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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