Reliable reference genes for expression analysis of proliferating and adipogenically differentiating human adipose stromal cells.
Krautgasser C., Mandl M., Hatzmann FM., Waldegger P., Mattesich M., Zwerschke W.
Laboratory Study, published in Cell Mol Biol Lett (2019) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Cell Mol Biol Lett (2019)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 30815013
- PMCID
- PMC6377720
- DOI
- 10.1186/s11658-019-0140-6
- Citations
- 11
Abstract (original English)
Background The proliferation and adipogenic differentiation of adipose stromal cells (ASCs) are complex processes comprising major phenotypical alterations driven by up- and downregulation of hundreds of genes. Quantitative RT-PCR can be employed to measure relative changes in the expression of a gene of interest. This approach requires constitutively expressed reference genes for normalization to counteract inter-sample variations due to differences in RNA quality and quantity. Thus, a careful validation of quantitative RT-PCR reference genes is needed to accurately measure fluctuations in the expression of genes. Here, we evaluated candidate reference genes applicable for quantitative RT-PCR analysis of gene expression during proliferation and adipogenesis of human ASCs with the immunophenotype DLK1 + /CD34 + /CD90 + /CD105 + /CD45 - /CD31 - . Methods We evaluated the applicability of 10 candidate reference genes ( GAPDH , TBP , RPS18 , EF1A , TFRC , GUSB , PSMD5 , CCNA2 , LMNA and MRPL19 ) using NormFinder, geNorm and BestKeeper software. Results The results indicate that EF1A and MRPL19 are the most reliable reference genes for quantitative RT-PCR analysis of proliferating ASCs. PSMD5 serves as the most reliable endogenous control in adipogenesis. CCNA2 and LMNA were among the least consistent genes. Conclusions Applying these findings for future gene expression analyses wi
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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