The Role of Macrophages in Implementing the Effects of Secretome of Mesenchymal Stromal Cells in the Spermatogonial Stem Cell Niche

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Postnatal stem cells surrounded by a niche support the renewal and regeneration of tissues and organs throughout life. The dysfunction of niche components can lead to the development of diseases, which are hard to cure. We previously showed that the subtunical injection of mesenchymal stromal cell (MSC) secretome induced the spermatogonial stem cell niche restoration, although the mechanisms of the process are not fully revealed. The study aimed at analyzing the effect of the MSC secretome on resident macrophages in animal models of male infertility and on peritoneal macrophages of intact animals. Materials and methods To study the effect of the MSC secretome on resident macrophages, doxorubicin-induced damage of murine spermatogenesis was modeled by intraperitoneal injections of 1 mg/kg of doxorubicin once in two days to reach a cumulative dose of 10 mg/kg. The second animal model of spermatogenesis injury was the abdominal cryptorchidism in rats. The MSC secretome was injected under the tunica albuginea. The animals were divided into the following groups: "intact", "damage", "MSC secretome". After isolating the testes, the number of macrophages was estimated using the immunohistochemical analysis. To investigate the phagocytic activity of macrophages mice were intramuscularly injected into the thigh with the MSC secretome with the following isolation of peritoneal macrophages

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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