Rolipram and Electrical Stimulation Synergistically Promote Neuronal Differentiation of Adipose-derived Stromal Cells: an in Vitro Study.
Rahimzadegan M., Khaboushan AS., Niknazar S., Ghahremani M., Javar HA., Sabzevari O.
Animal Study, published in Stem Cell Rev Rep (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Stem Cell Rev Rep (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40563059
- PMCID
- PMC12408716
- DOI
- 10.1007/s12015-025-10925-5
- Citations
- 1
Abstract (original English)
This study investigates gold-decorated polycaprolactone/chitosan nanofibers as conductive scaffolds for promoting neuronal differentiation of adipose-derived mesenchymal stromal cells (ADSCs) harvested from the dorsal interscapular region of Wistar rats under electrical stimulation (ES) with optimal rolipram concentrations. The scaffold was fabricated through electrospinning and in situ synthesis of gold nanoparticles (AuNPs). Morphology and AuNPs distribution were evaluated using a Field Emission Scanning Electron Microscope (FE-SEM) and energy dispersive X-ray spectroscopy (EDX). Rolipram, known to increase neuronal cyclic adenosine monophosphate (cAMP) activity and reduce inflammation, was loaded into the scaffolds using alginate hydrogel. The scaffolds were subjected to a release study and tests for ADSC proliferation and differentiation into neuron-like cells. Immunostaining of β-Tubulin III and MAP2 was used to assess the effect of ES, alone and in combination with rolipram, on the efficacy of neuronal differentiation of ADSCs. The distribution of AuNPs was uniform within the scaffolds with an electrical conductivity of 0.12 S.cm -1 . Rolipram significantly improved the development of neurons from ADSCs, and this effect was more prominent at higher concentrations (1 and 5 µM). The study revealed that using an electrical density of 100 mV/mm, in combination with 5 µM rolip
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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