Secreted Peptidome Profiling of Human Dental Pulp Stem Cells-Conditioned Medium During Odontogenic Differentiation
Huang Y., Liu S., Wu H., Zhang Z., Chen H., Xie K.
Laboratory Study, published in Int Dent J (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Int Dent J (2026)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 42155947
- PMCID
- PMC13213307
- DOI
- 10.1016/j.identj.2026.109616
Abstract (original English)
Introduction and aims Odontogenic differentiation of human dental pulp stem cells (hDPSCs) is critical for pulp repair. This study aims to characterise the secretory peptidome of human dental pulp stem cells (hDPSCs) during odontogenic differentiation, thereby providing insights into potential dental pulp repair strategies. Methods We employed tandem mass tag labelling coupled with liquid chromatography-tandem mass spectrometry to compare the peptide profiles of undifferentiated and differentiated hDPSCs-conditioned medium (CM), followed by in-depth bioinformatics analysis. A Cell Counting Kit-8 assay was conducted to screen for functional peptides present in the hDPSCs-CM. 5-Ethynyl-2'-deoxyuridine staining, flow cytometry, transwell migration, alkaline phosphatase activity, and alizarin red S staining, quantitative real-time polymerase chain reaction, and Western blot analyses were performed to preliminarily investigate the functions of the selected peptide. Results A total of 90 peptides were differentially expressed in differentiated hDPSCs-CM compared with undifferentiated hDPSCs-CM. Gene Ontology analysis suggested that the differentially expressed peptides were closely associated with biological processes, including cell proliferation, growth, and reproduction. Kyoto Encyclopedia of Genes and Genomes enrichment analysis suggested that the precursor proteins of these pept
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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