SVF
Level D· Preclinical EvidenceAnimal Study

Self-assembled hADSCs/hNSCs spheroids combined with 3D printed M-shaped GelMA/Pu Scaffolds: Creating histologically biomimetic engineered cartilage that meets the characteristics of alar cartilage.

Wang G., Lin W., Yan W., Zhang C., Dang W., Meng X.

Animal Study on Face & Skin, published in Biomaterials (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Animal Study
Journal
Biomaterials (2026)
Country
Netherlands
Reported sample size
—
PMID
40946483
DOI
10.1016/j.biomaterials.2025.123691

Abstract (original English)

Traditional cartilage grafts for nasal alar retraction repair often lack sufficient elasticity and histocompatibility, leading to suboptimal clinical outcomes. To address this challenge, we developed a biomimetic engineered cartilage scaffold that combines self-assembled human adipose-derived stem cell (hADSC)/human nasal septal chondrocyte (hNSC) spheroids with a 3D-printed M-shaped polyurethane (Pu)/gelatin methacryloyl (GelMA) hybrid scaffold, aiming to replicate the extracellular matrix (ECM) components and mechanical properties of native alar cartilage. The M-shaped scaffold was designed based on clinical CT reconstruction and fabricated via 3D printing using Pu for optimal elasticity. hADSCs and hNSCs were co-cultured at varying ratios (0:5-5:0) and loaded as spheroids into GelMA-coated scaffolds. ECM components (Col-1, Col-2, ACAN, Elastin) were analyzed via RT-qPCR and immunohistochemistry. Subcutaneous (nude mice) and in-situ nasal (sheep) implantation models evaluated chondrogenic performance, ECM deposition and structural integration over 1-2 months. Through comparative analysis of extracellular matrix (ECM) components among human alar cartilage, nasal septal cartilage, auricular cartilage, and costal cartilage, we identified the target ECM profile for biomimetic alar cartilage engineering. It was verified that self - assembled spheroids have stronger chondrogenic ab

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence
HumansTissue ScaffoldsAnimalsTissue EngineeringPrinting, Three-DimensionalSpheroids, CellularPolyurethanesChondrocytesExtracellular MatrixGelatin
Face & Skin
View on PubMedDOI

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