Sendai virus-based human transcriptional gene delivery system fails to reprogram bovine mesenchymal stromal cells (MSC) into induced pluripotent stem cells (iPSC).
Quiroga B., Leiva B., Díaz C., Morera FJ., Paiva L., Torres CG.
Animal Study with a reported sample of 9, published in Mol Biol Rep (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Mol Biol Rep (2026)
- Country
- Netherlands
- Reported sample size
- 9
- Source database
- PubMed
- PMID
- 42171938
- DOI
- 10.1007/s11033-026-11961-1
Abstract (original English)
Despite the relevance of induced pluripotent stem cells (iPSCs) technology for biotechnological applications in cattle, the generation of bona fide bovine iPSCs (biPSCs) remains challenging. This study aimed to evaluate the use of a non-integrating Sendai virus system carrying polycistronic human KLF4-OCT3/4-SOX2, c-MYC, and KLF4 to reprogram bovine fetal mesenchymal stem/stromal cells (MSCs) derived from adipose tissue (AT-MSCs) and bone marrow (BM-MSCs) into biPSCs. AT-MSCs, BM-MSCs, and control fibroblasts (FBs) were isolated from bovine fetuses (n = 9; 8 months of gestation). A CytoTune™-iPS 2.0 Sendai kit was used for reprogramming AT-MSCs, BM-MSCs and FBs. Cell culture samples were collected on days 0, 7, and 28 post-transduction for pluripotency and epigenetic gene expression analysis by quantitative PCR (qPCR) and on day 28 for assessment of pluripotency markers by immunofluorescence and alkaline phosphatase (AP) activity. Cells formed colonies with undefined margins and low AP activity. Endogenous bovine OCT4, SOX2, and NANOG proteins were detected in the nuclei and cytoplasm of FBs, AT-MSCs, and BM-MSCs. However, OCT4 gene expression was detected only in FBs. Gene expression of DNA methyltransferase DNMT3B and histone demethylase KDM6 were increased (P < 0.05) in AT-MSCs and FBs, respectively. Insufficient activation of core pluripotency (i.e., endogenous OCT4) and un
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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