Senescence Detection Using Reflected Light in Adipose Stromal Vascular Fraction.
Westerberg LJS., Barbe L., Ehrström M., Spalding KL.
Laboratory Study, published in J Vis Exp (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- J Vis Exp (2026)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42330043
- DOI
- 10.3791/70588
Abstract (original English)
Cellular senescence is a stress-induced state characterized by permanent cell-cycle arrest and the development of a distinctive secretory profile that impacts tissue function and contributes to aging and metabolic disease. Senescence-associated β-galactosidase (SA-β-gal) activity is widely used as a marker of senescent cells; however, conventional SA-β-gal assays often rely on subjective visual assessment and provide limited quantitative information. These limitations are particularly evident in primary human cell populations such as the stromal vascular fraction (SVF) derived from adipose tissue, which contains a heterogeneous mixture of preadipocytes, immune cells, and endothelial cells. Here, we present an optimized reflected light confocal microscopy approach for high-resolution, quantitative detection of SA-β-gal activity in human SVF cells. This protocol enables objective single-cell analysis of SA-β-gal activity, allows simultaneous immunocytochemistry for multiplexed detection of additional senescence or lineage markers, and incorporates pH-matched controls. By combining these features, this method provides a sensitive, reproducible, and quantitative approach to studying cellular senescence in heterogeneous primary human cell populations. It offers an improved alternative to conventional SA-β-gal staining.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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