Setup of an In Vitro Three-Dimensional Stromalized Prostate Cancer Model Using Gelatin Microparticles
Gangarossa G., Iozzo M., Mugnaini G., Gelli R., Ippolito L., Giannoni E.
Laboratory Study, published in ACS Omega (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- ACS Omega (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 40521560
- PMCID
- PMC12163836
- DOI
- 10.1021/acsomega.5c01286
Abstract (original English)
Developing three-dimensional (3D) tumor models that accurately mimic the tumor microenvironment (TME) and its heterogeneity remains a significant challenge in preclinical research. Advancing these models holds the potential to improve the study of cancer pathologies in vitro, while reducing dependence on animal models. To tackle this challenge, in this work, we report on the development of an in vitro 3D stromalized prostate cancer model using gelatin porous microparticles as microscaffolds for cell attachment and growth. Gelatin porous microparticles were prepared by a double emulsion method and cross-linked with a biocompatible cross-linking agent, that is, glyceraldehyde, to prevent dissolution under physiological conditions. Then, we developed a stromalized 3D gelatin-based microscaffold biomimicking the interplay between human prostate cancer (PCa) and stromal cells by coculturing 22Rv1 cells and fibroblasts with gelatin porous microparticles. Overall, our results demonstrate the feasibility of gelatin microscaffolds in reproducing a 3D stromalized model of PCa progression (e.g., metabolic reprogramming), resulting from the tumor-stroma interaction. Thus, these systems represent a valuable platform and an effective tool for the study of cancer progression, such as TME biomimetics, while simultaneously offering a valid alternative to minimize the reliance on animal studies
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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