Silk Fibroin Scaffold-Based 3D Co-Culture Model for Modulation of Chondrogenesis without Hypertrophy via Reciprocal Cross-talk and Paracrine Signaling.
Bhardwaj N., Singh YP., Mandal BB.
Laboratory Study, published in ACS Biomater Sci Eng (2019) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- ACS Biomater Sci Eng (2019)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 33455229
- DOI
- 10.1021/acsbiomaterials.9b00573
Abstract (original English)
In this study, the effect of cellular cross-talk on modulation of chondrogenesis and hypertrophy while minimizing the usage of articular chondrocytes (ACs) has been investigated. Herein, co-culture of ACs with adipose-derived human mesenchymal stem cells (ADhMSCs) was employed for cross-talk within silk fibroin (SF)-based three-dimensional (3D) scaffolds. The co-culture model was developed by co-culturing four different ratios of ADhMSCs to ACs: 1:0, 3:1, 1:1, and 0:1 on porous 3D SF scaffolds for 21 days. The co-culture groups were cultured in defined media without adding any exogenous growth factors except the monoculture group, ADhMSC-only controls. The co-cultured constructs indicated significantly higher cellular viability and proliferation than the control monoculture groups. The supernatants of co-culture groups indicated significantly higher levels of TGF-β1 and IL-10, which confirmed the production of the morphogens/signaling molecules by chondrocytes for induction of ADhMSCs differentiation toward the chondrogenic phenotype. Biochemical assays indicated enhanced accumulation of sulfated glycosaminoglycans, collagen, and high DNA content along with high cellularity in co-culture groups than chondrocyte-only controls. Co-culture groups revealed synergistic interactions between cells as indicated by the interaction index value ranging from 2-3. Furthermore, upregulation
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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