Single-cell RNA and ATAC sequencing reveals cell subsets characteristics in adipose-derived stem cells.
Long Q., Yuan Y., Liu Z., Zhang P., Yuan X.
Laboratory Study, published in Biochim Biophys Acta Mol Cell Biol Lipids (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Biochim Biophys Acta Mol Cell Biol Lipids (2026)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41724372
- DOI
- 10.1016/j.bbalip.2026.159734
Abstract (original English)
The inherent heterogeneity of adipose-derived stem cells (ADSCs) complicates their characterization, as aggregated data obscure the nuanced states of individual cells and are skewed by dominant functional subpopulations affecting genetic and biological profiles. This heterogeneity presents a significant challenge for the effective deployment of ADSCs in clinical and research settings, highlighting the necessity for precise identification and isolation of specific subsets according to stringent criteria. Using single-cell RNA + ATAC multi-omics technology, we sequenced ADSCs derived from human adipose tissue through extraction, culture, and purification. This analysis identified three distinct subsets-proliferative, functional, and senescent-each exhibiting unique stemness properties. Notably, within the functional subset, cluster 6 emerged as a prime candidate for cellular engineering, showcasing robust stemness, high proliferative capacity, and low senescence. Our findings also reveal ADSCs' predisposition toward neuro-lineage differentiation, with their spatial distribution reflecting developmental trajectories and biological functions. In-depth analysis uncovered subset-specific genes with unique chromatin accessibility patterns, critical for targeted differentiation. Significantly, stemness markers BNC2 and HMGA2 were identified as indicators of non-senescent ADSCs. Through
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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