Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition.
Wang L., Liu S., Zhang S., Wang Y., Zhou Y., Shan T.
Animal Study, published in Elife (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Elife (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40053468
- DOI
- 10.7554/eLife.99790
Abstract (original English)
Conjugated linoleic acids (CLAs) can serve as a nutritional intervention to regulate quality, function, and fat infiltration in skeletal muscles, but the specific cytological mechanisms remain unknown. Here, we applied single-nucleus RNA-sequencing (snRNA-seq) to characterize the cytological mechanism of CLAs regulates fat infiltration in skeletal muscles based on pig models. We investigated the regulatory effects of CLAs on cell populations and molecular characteristics in pig muscles and found CLAs could promote the transformation of fast glycolytic myofibers into slow oxidative myofibers. We also observed three subpopulations including SCD + /DGAT2 + , FABP5 + /SIAH1 + , and PDE4D + /PDE7B + subclusters in adipocytes and CLAs could increase the percentage of SCD + /DGAT2 + adipocytes. RNA velocity analysis showed FABP5 + /SIAH1 + and PDE4D + /PDE7B + adipocytes could differentiate into SCD + /DGAT2 + adipocytes. We further verified the differentiated trajectory of mature adipocytes and identified PDE4D + /PDE7B + adipocytes could differentiate into SCD + /DGAT2 + and FABP5 + /SIAH1 + adipocytes by using high intramuscular fat (IMF) content Laiwu pig models. The cell-cell communication analysis identified the interaction network between adipocytes and other subclusters such as fibro/adipogenic progenitors (FAPs). Pseudotemporal trajectory analysis and RNA velocity analysis al
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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