Single step fabrication of muscle bundles for cultivated meat using mechanotransduction: a paradigm shift.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Cultivated meat (CM) has emerged as a sustainable alternative to traditional livestock production however, commercialization remains constrained by several interconnected challenges, including the high costs of chemical differentiation media, and the complexity of multi-step scaffold-cell assembly processes. Cell expansion cost is an additional challenge that will be addressed by complementary efforts in the field. Here, we present a novel single-step, food-compatible wet-spinning platform that simultaneously integrates scaffold fabrication, stem cell encapsulation, and mechanotransducive stimulation, eliminating the need for exogenous soluble differentiation factors and post-fabrication cell seeding. This platform is fundamentally distinct from multi-step scaffold-cell assembly workflows reported in the literature, where scaffold fabrication, surface modification, cell seeding and biochemical myogenic differentiation induction are performed as sequential, independent operations. Using porcine adipose-derived stem cells (pADSCs) encapsulated within alginate-gelatin composite hydrogel microfibers, we demonstrate that scaffold stiffness and fabrication-induced shear stress alone are sufficient to drive myogenic progression toward terminal differentiation without any biochemical inducers. By harnessing mechanotransduction as the primary differentiation driver, this approach addres

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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