Small extracellular vesicles produced by homeostatic phenotype BMSC promote bone regeneration by transferring anti-senescent factor JMJD6
Fu S., Liu L., Xu F., Cheng J., Liu Y., Liu T.
Animal Study, published in J Nanobiotechnology (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Nanobiotechnology (2026)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41723429
- PMCID
- PMC13032466
- DOI
- 10.1186/s12951-026-04199-3
- Citations
- 1
Abstract (original English)
The therapeutic efficacy of extracellular vesicles (EVs) depends on the status of their donor cells. Cellular senescence of bone marrow mesenchymal stem cells (BMSCs), which induces significant changes to cellular secretome, greatly affects the osteogenesis of the donor BMSCs along with BMSC-derived EVs. Stem cells prefer a homeostatic self-renewing state to alleviate the senescence and loss of stemness caused by external disturbance, but which inevitable in EV produce. In this study, to avoid the senescence of BMSCs caused by in vitro culture and boost the osteogenesis efficacy of BMSC-derived small extracellular vesicles (sEVs), we established a niche-mimicking (NM) culturing system to maintain the cellular homeostasis that significantly delayed stem cell senescence in vitro. As a result, single cell transcriptome revealed that NM-culturing significantly enhanced the expression level of the homeostasis-related genes. Then we found that sEVs produced by homeostatic BMSCs exhibited superior osteogenic stimulation efficacy and bone defect repair compared to those from conventional monolayer-cultured BMSCs. Mechanically, by analyzing the "shuttling effect" of sEVs via multi-omic analysis, we found that the key homeostasis-related genes (including JMJD6, LIF, CYP19A1, and LAMA1) functioned throughout the entire process, by thus we defined the "homeostatic phenotype" of BMSC. Among
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.