Small molecule cocktail for the serum-free Adipogenic induction of adipose-derived stem cells from Larimichthys crocea: Towards the efficient production of cell-cultured fish fat.
Zhou X., Wei Y., Meng Z., Feng Y., Li Z., Zheng H.
Animal Study, published in Food Res Int (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Food Res Int (2025)
- Country
- Canada
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41508513
- DOI
- 10.1016/j.foodres.2025.118109
Abstract (original English)
This study aims to develop a safe, efficient, and serum-free induction platform for the adipogenic differentiation of adipose-derived stem cells (ASCs) from Larimichthys crocea, to advance the production of cultivated fat for cellular agriculture. Through systematic screening of plant-derived small molecules, bavachinin and dehydroabietic acid were identified as core inducing components, with their concentrations and exogenous lipid supplementation strategies optimised. A serum substitute culture system (SS) was successfully established, comprising growth factors, antioxidants, and carrier proteins, which effectively supports ASCs adipogenesis. Further investigation under three-dimensional culture on gelatin-based porous microcarriers revealed that lower stiffness (10 kPa) enhanced lipid accumulation and adipogenic gene expression. Transcriptomic and metabolomic analyses indicated that the induction system activates the PPARγ pathway and promotes lipid synthesis and accumulation. This work provides a robust chemical biology strategy and theoretical foundation for the scalable production of cultivated fish adipose tissue.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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