Stromal Vascular Fraction-Derived Vasculogenesis Is Associated with the Formation of Lymphatic Endothelial Cell Structures.
Hu NW., Shang H., Kogan S., Llull R., Traktuev DO., Katz AJ.
Animal Study, published in Stem Cells Dev (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Stem Cells Dev (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40533090
- DOI
- 10.1089/scd.2024.0210
- Citations
- 4
Abstract (original English)
Therapies aimed at manipulating microvasculature require the ability to generate both blood and lymphatic vessels. Adipose-derived stromal vascular fraction (SVF), consisting of endothelial cells, progenitor cells, pericytes, smooth muscle cells, fibroblasts, and immune cells, has emerged as a heterogeneous cell composition able to promote blood vessel formation and growth, but whether SVF forms lymphatic vessels remains unknown. The objective of this study was to evaluate whether SVF can form lymphatic vessels. SVF was isolated from C57BL/6 mouse inguinal adipose tissue, characterized for prevalence of blood (PECAM+) and lymphatic (Prox1+, Podoplanin+, LYVE-1+) endothelial cells and cultured with avascular mouse mesentery tissues for up to 9 days. The presence of lymphatic endothelial cells in SVF is supported by the percentages of PECAM+ cells that are also positive for lymphatic markers. By day 1 after SVF seeding, cells established PECAM+ segments, and by day 3 cell clusters with segment extensions were observed. At later time points, segments established network of blood vessels. In parallel, a subset of structures positive for lymphatic marker labeling and characterized by a rounded shape (termed "blebs") connected with nearby SVF-derived blood vessel and were changing shape over time. Our findings provoke a new research area focused on the ability for SVF to form lymphat
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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