Superficial and deep zone articular chondrocytes exhibit differences in actin polymerization status and actin-associated molecules in vitro
Delve E., Co V., Kandel RA.
Laboratory Study, published in Osteoarthr Cartil Open (2020) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Osteoarthr Cartil Open (2020)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 36474687
- PMCID
- PMC9718196
- DOI
- 10.1016/j.ocarto.2020.100071
- Citations
- 10
Abstract (original English)
Objective The actin cytoskeleton regulates cell shape and plays a role in regulating chondrocyte phenotype. Most studies investigating regulation of the chondrocyte phenotype by the actin cytoskeleton use chondrocytes isolated from full-thickness (FT) cartilage, which has a heterogeneous cell population. Superficial zone chondrocytes (SZC) have an elongated morphology and account for 10-20% of chondrocytes, while the remaining chondrocytes in the deeper zones appear more rounded. This study characterizes the actin cytoskeleton and expression of actin-associated molecules in SZC and deep zone (DZ) chondrocytes (DZC) in vitro in order to identify molecules differentially expressed by SZC and DZC that may contribute to the observed differences in zonal chondrocyte shapes. Design SZ, DZ, and FT chondrocytes isolated from bovine metacarpal-phalangeal joints were cultured in monolayer for 48 h . Macroscopic morphology, actin polymerization status, and expression of select actin-associated molecules (adseverin, cofilin, transgelin, vinculin, MRTF-A, and YAP/TAZ) were determined. Results SZC appeared more elongated and have more filamentous actin compared to DZC, as determined by quantifying cell circularity and G-/F-actin ratio. MRTF-A gene and protein levels were significantly higher in SZC compared to DZC while DZC more highly expressed transgelin and TAZ. Although there was differe
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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