Synergistic effects of thermal treatment and encapsulation of calcium phosphate nanoparticles on enhancing dimensional stability and osteogenic induction potential of free-standing PLGA electrospun membranes.
Shi M., Xuan L., Zhang Y., Wang D., Ye F., Shi X.
Animal Study, published in Colloids Surf B Biointerfaces (2019) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Colloids Surf B Biointerfaces (2019)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 31445359
- DOI
- 10.1016/j.colsurfb.2019.110437
- Citations
- 7
Abstract (original English)
Limited dimensional stability and osteogenic induction property of poly (lactide-co-glycolide) (PLGA) electrospun membranes hampered their applications in bone tissue engineering. Thermal treatment of fixed PLGA membranes at 50 °C for 2 h and further immersion in 75% ethanol at free-standing state were adopted in order to obtain a high stability and well-maintained fiber morphology. After the process, free-standing membranes were stable during incubation in PBS at 37 °C, the volumetric ratio was 59.0%, fibers became curved, and the average diameter was 816 nm. For as-electrospun PLGA membranes, the volumetric ratio was only 35.3%, showing that thermal treatment was effective to improve the dimensional stability. The addition of calcium phosphate nanoparticles in P5-T-F further increased the volumetric ratio (64.0%) and significantly improved the mechanical properties. The mineralization capacity of PLGA membranes was enhanced because of thermal treatment. Hemolysis ratios of all samples were ∼2% indicating good hemocompatibility of PLGA electrospun membranes. Proliferation of adipose derived stem cells from rats (rADSCs) on treated PLGA membranes was significantly faster than that on untreated one, especially for sample P-T-F. In addition to thermal treatment, the addition of calcium phosphate nanoparticles showed synergistic effects on improving mineralization property and ost
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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