Targeting senescent cells in aged adipose tissue induced by ovarian cancer with the NF-κB inhibitor quercetin and resveratrol impedes the growth and metastasis of ovarian cancer.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

As a lipophilic tumour, ovarian cancer (OC) preferentially metastasizes to adipose-rich environments including the omentum and the peritoneum. Current research regarding the OC microenvironment has focused primarily on tumour-associated immune cells, whereas little attention has been given to the adipose tissue and the adipose-derived stem cells (ADSCs) within it. Here, from the perspective of senescence, we hypothesized that the continuous presence and accumulation of tumour cells disrupts the homeostasis of adipose tissue by intercellular interactions. Through a series of in vitro and in vivo experiments, we found that OC cells induce adipose tissue ageing and ADSC senescence, leading to adipose tissue dysfunction, glucose intolerance, and insulin resistance. OC extracellular vesicle (OC-EV) and ADSC coculture revealed that OC-EVs trigger ADSC ageing and dysfunction. Moreover, coculture promoted the formation of inflammasomes in ADSCs and increased the levels of the inflammatory factors IL-1β and IL-18. RNA-sequencing and bioinformatics analysis revealed that the nuclear factor kappa B (NF-κB) signalling pathway is involved in ADSC senescence induced by OC cells. Immunofluorescence staining and Western blotting confirmed that increased expression of NF-κB signalling-related proteins is associated with senescent ADSCs. Further mechanistic studies revealed that OC-EVs deliver I

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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