Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation
Côté JA., Guénard F., Lessard J., Lapointe M., Biron S., Vohl MC.
Prospective Study with a reported sample of 4, published in Int J Genomics (2017) — summary generated from the PubMed abstract.
Early human evidence such as case series or small samples is exploring possible benefits.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Prospective Study
- Journal
- Int J Genomics (2017)
- Reported sample size
- 4
- Source database
- Europe PMC
- PMID
- 28409151
- PMCID
- PMC5376413
- DOI
- 10.1155/2017/5149362
- Citations
- 16
Abstract (original English)
Objective . To characterize changes in gene expression profile during human mature adipocyte dedifferentiation in ceiling culture. Methods . Subcutaneous (SC) and omental (OM) adipose tissue samples were obtained from 4 participants paired for age and BMI. Isolated adipocytes were dedifferentiated in ceiling culture. Gene expression analysis at days 0, 4, 7, and 12 of the cultures was performed using Affymetrix Human Gene 2.0 STvi arrays. Hierarchical clustering according to similarity of expression changes was used to identify overrepresented functions. Results . Four clusters gathered genes with similar expression between day 4 to day 7 but decreasing expression from day 7 to day 12. Most of these genes coded for proteins involved in adipocyte functions ( LIPE , PLIN1 , DGAT2 , PNPLA2 , ADIPOQ , CEBPA , LPL , FABP4 , SCD , INSR , and LEP ). Expression of several genes coding for proteins implicated in cellular proliferation and growth or cell cycle increased significantly from day 7 to day 12 ( WNT5A , KITLG , and FGF5 ). Genes coding for extracellular matrix proteins were differentially expressed between days 0, 4, 7, and 12 ( COL1A1 , COL1A2 , and COL6A3 , MMP1 , and TGFB1 ). Conclusion . Dedifferentiation is associated with downregulation of transcripts encoding proteins involved in mature adipocyte functions and upregulation of genes involved in matrix remodeling, cellula
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
Evidence level
Early human evidence such as case series or small samples is exploring possible benefits.
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