Therapeutic Efficacy of Adipose-Derived Stem Cells for Bleomycin-Induced Idiopathic Pulmonary Fibrosis: A Comparative Study of Intravenous versus Intratracheal Administration.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objective To compare the therapeutic effects of intravenous (IV) and intratracheal (IT) delivery of adipose-derived stem cells (ADSCs) in a mouse model of bleomycin (BLM)-induced idiopathic pulmonary fibrosis (IPF). Materials and methods In this experimental study, BALB/c mice were divided into 4 groups: control, BLM only, BLM+ADSC(IV), BLM+ADSC(IT). ADSCs were administered on day 14 after BLM induction. Outcomes measured on day 42 included mortality, body weight, lung edema, collagen deposition, fibrosis scoring, cytokine levels (TGF-β, SDF-1, IL-6, IL-1β, TNF-α), hydroxyproline, and leukocyte infiltration. Results ADSC(IT) reduced mortality more effectively than ADSC(IV). Both IV and IT mitigated weight loss and lung edema with no significant difference. Both routes significantly decreased collagen synthesis, collagen fiber accumulation, Ashcroft scores, and hydroxyproline levels. Both routes lowered TGF-β, α-SMA, TNF-α, IL-6, IL-1β, and SDF-1 levels, and reduced leukocyte infiltration and alveolar barrier dysfunction, with no significant difference between routes. Conclusion This design enables discrimination between route-dependent and route-independent therapeutic ef fects of ADSCs, thereby providing new insight into the optimization of stem cell delivery strategies for pulmo nary fibrosis. Importantly, whether the choice of deliv ery route differentially affects survival

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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