Three-Dimensional Influence of Photobiomodulation on Adipose-Derived Stem Cell Behavior.
Mulaudzi PE., Abrahamse H., Crous A.
Laboratory Study, published in J Biophotonics (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- J Biophotonics (2025)
- Country
- Germany
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41339125
- PMCID
- PMC12977305
- DOI
- 10.1002/jbio.202500414
- Citations
- 3
Abstract (original English)
Photobiomodulation (PBM) a promising non-invasive therapeutic technique has been proposed to have regenerative ability. There is limited understanding of PBMs effectiveness in 3D cell culture systems. To evaluate the effect of PBM at 825 nm on adipose-derived stem cells (ADSCs) cultured in 2D and 3D spheroid models. ADSCs were exposed to PBM at fluences of 5, 10, and 15 J/cm 2 to assess cellular responses. Cell morphology, spheroid diameter, cell concentration, viability, proliferation, and cytotoxicity were monitored 24- and 72-h post-irradiation (hpi). In 2D cell culture, PBM at 5 J/cm 2 showed a significant increase in cell viability and proliferation. Cellular cytotoxicity was higher in 2D. Spheroids maintained steady size, increased viability, and proliferation peaking at 24 hpi but remaining stable over time at 10 J/cm 2 . 2D cell cultures show an over expression of PBM-induced effects, while spheroids provide a more physiological and balanced response profile particularly at 10 J/cm 2 .
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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